KEY FEATURES:
- Label-free, kinetic quantification of neurite dynamics (initiation, branching, extension, retraction)
- Automated image acquisition and integrated analysis software facilitate accurate neurite detection and data analysis
- Multiplexed assay capability for parallel monitoring of HD phase and multi-color fluorescent images using the IncuCyte ZOOMTM
- Compatible with multiple neuronal cell types (cell lines and primary cells)
Neurite outgrowth of rat E18 cortical neurons in a 96-well microplate. A) 20x HD phase image of primary neurons, 96 hours post plating ; B) Image segmentation of neurites (yellow) and cell body cluster (raspberry); C) Concentration and time dependent inhibition of neurite outgrowth with the protein kinase C inhibitor, Ro-31-8220 (mean ± SD; n=6 per condition).
Neurite outgrowth of Neuro-2a NucLight Red cells in a 96-well microplate. Left:
10x blended HD phase and red fluorescence image 78 hours
after treatment with 20 µM all-trans retinoic acid; Right: Concentration dependent increase in neurite length with all-trans retinoic acid measured at 78 hours post treatment; neurite length normalized to nuclear count (mean ± SD; n=10).
Neurite outgrowth of iPSC-derived iCell Neurons in a 96-well microplate. Left: Neurite outgrowth of iCell neurons at varying densities (mean ± SD; n=6 per condition); Right: 20x HD phase image of iCell Neurons at 10k cells/well at 96 hours.
iCell® is a registered trademark of Cellular Dynamics International.